Cyclic AMP-binding proteins and protein kinase during regression of Walker 256 mammary carcinoma.

Loss of nuclear cyclic AMP binding in cyclic AMP-unresponsive Walker 256 mammary carcinoma.

A marked increase of cyclic AMP-binding and protein kinase activities occurs in the nuclei of N6,02’-dibutyryl adenosine 3’:5’-monophosphate (Bt,cAMP)-responsive Walker 256 mammary carcinoma (W256) following incubation of the tumor slices with CAMP in uitro. The macromolecular fraction containing [“HIcAMP in the nuclei can be extracted with 1.0 M KC1 and identified by acrylamide gel electrophor...

Walker Carcinoma 256

Progressive growth of the Walker carcinoma @56 produces a caloric deficit in young adult, male, albino rats. The excessive expenditure of calories is not due solely to decreased food intake, for tumor-bearing rats lose more calories than do pair-fed noncancerous rats of the same age, sex, and initial weight. This result was anticipated by a previous report that rats in which the Walker tumor gr...

Walker Carcinoma 256*t

Dieta.—Male Wistar rats were placed on a semi synthetic diet at weaning, and the food consump tion of each was measured. A week later each rat was paired with one of equal body weight and food consumption. At this time one rat of each pair was designated as the experimental rat which would receive a tumor transplant; the other rat, his pair fed control, consumed the amount of diet eaten by th...

Title 3 ' : 5 ' - Cyclic AMP Binding Proteins

Cyclic AMP-binding and cyclic AMP-dependent protein kinase activities in the cytosol of differentiating bone marrow erythroblasts.

Cytosolic cyclic AMP-binding capacity and cyclic AMP-dependent protein kinase activity have been studied in relation to differentiation and maturation of rabbit bone marrow erythroblasts. Using cells fractionated by velocity sedimentation at unit gravity, it was found that both activities decreased in dividing cells when calculated in terms of cell number but remained constant per cell volume. ...